Journal: Communications Biology

Article Title: AKAP150-anchored PKA regulates synaptic transmission and plasticity, neuronal excitability and CRF neuromodulation in the mouse lateral habenula

doi: 10.1038/s42003-024-06041-8

Figure Lengend Snippet: Example of a brain section stained with antibody to AKAP150 (green), showing the expression of AKAP150 in the mouse LHb. Scale bar, 20 μm.

Article Snippet: Serial coronal sections (20 μm) of the midbrain containing the LHb (from −2.64 to −4.36 mm caudal to bregma (Paxinos and Watson, 2007) were fixed in 4% PFA for 5 min, washed in 1x PBS, and then blocked in 10% normal goat serum containing 0.3% Triton X-100 in 1x PBS for 1 h. Sections were incubated in goat anti- AKAP150 antibody (1:500, Santa Cruz Sc-6445) in carrier solution (5% normal goat serum in 0.1% Triton X-100 in 1x PBS) overnight at room temperature.

Techniques: Staining, Expressing

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Expression of AKAP150 upregulated in the NAc after naloxone-precipitated morphine withdrawal (A–C) Schematic of the three-compartment apparatus applied for the CPA test. (B and C) Experimental timeline of the morphine withdrawal physical signs test (B) and the morphine withdrawal CPA model (C). (D) Morphine withdrawal physical signs test [jumps (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), paw tremor (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, Mann-Whitney U test, n = 10)] and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly increased after morphine withdrawal in WT mice. (E) Less time was spent in the paired compartment after the morphine withdrawal CPA model was established. CPA aversion score was significantly reduced after morphine withdrawal in WT mice (∗∗∗ P <0.001, t test, n = 10). (F) Mean velocity showed no significant difference before and after morphine withdrawal (Pre-paired, p = 0.6961, t test; Post-paired, p = 0.7676, t test; n = 10). (G–I) AKAP150 mRNA (G) and protein expression (H and I) were upregulated in morphine withdrawal model by PCR, IHC, and WB test (∗∗∗ P <0.001, t test, n = 6). (J). IHC showing c-fos expression was upregulated after morphine withdrawal. Scale bar, 100μm. (K) Representative images of GFP-labeled dendrites and the statistical data for density of dendritic spines, demonstrating the density of dendritic spine was significantly higher after morphine withdrawal (∗∗∗ P <0.001, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and CPA score in WT mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗∗ P <0.01, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, t test, n = 10) were decreased, and morphine withdrawal score (∗∗ P <0.01, Mann-Whitney U test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in WT mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in WT mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in WT mice. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in WT mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Viruses used in the study

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: shRNA

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and aversion score in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased, and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice morphine withdrawal model. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: AKAP150 was highly expressed in D1R-MSNs and D2R-MSNs (A) UMAP plot of 35,878 single cells grouped into 19 major cell types. Each dot represents one single cell, colored according to cell type. (B) Feature plot of the normalized expression of AKAP150 gene for each cell type and the depth of color from gray to blue represents low to high expression. (C) Violin plots showed the normalized expression of AKAP150 gene (rows) in each cell cluster (columns). Cell clusters and the expression level of AKAP150 are indicated at the x and y axis, respectively. (D) Violin plots showed the normalized expression of AKAP150 gene (rows) from one time morphine-treated mice and saline-treated mice (columns) in D1R-MSN cluster (left ), D2R-MSN cluster (middle ) and GABA_other cluster (right ). Treat group and the expression level of AKAP150 are indicated at the x and y axis, respectively. p values are derived from two-sided Wilcoxon test. (E) Level of AKAP150 mRNA relative expression was not significantly changed after one time treatment of morphine compared with saline (N.S. = no significance, p > 0.05, t test, n = 6). (F and G) IHC showed expression of AKAP150 in D1 (F) and D2 (G) receptor positive neuron in the naloxone-precipitated morphine withdrawal mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Expressing, Saline, Derivative Assay

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in WT mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in WT mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗∗ P <0.001, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in WT mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic responses including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). (G) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. (H) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Activity of AKAP150 expressed in D1R-MSNs and D2R-MSNs in NAc received mediation from VTA (A and B) AAV1-TH-Cre in VTA promoted the expression of CMV-DIO-shRNA-EGFP in NAc and relieved the morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗ P <0.05, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower (A) as well as the CPA scores (∗∗∗ P <0.001, t test, n = 10) (B). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in the NAc mediated by VTA (∗∗ P <0.01, t test, n = 6). (D and E) Inhibition of AKAP150 in D1R-MSNs receiving projections from VTA relieved the morphine withdrawal aversion (∗∗ P <0.01, t test, n = 10) (E) without relieving morphine withdrawal somatic signs (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10) (D). (F) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D1R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). (G and H) Inhibition of AKAP150 in D2R-MSNs receiving projections from VTA relieved the morphine withdrawal somatic signs [jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, t test, n = 10), loss of body weight (∗∗ P <0.01, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10)] (G) without relieving the morphine withdrawal aversion (N.S. = no significance, p > 0.05, t test, n = 10) (H). (I) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D2R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Activity Assay, Expressing, shRNA, MANN-WHITNEY, Knockdown, Inhibition

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet:

Article Snippet: Primary antibodies against AKAP150 (Santa Cruz, Cat# sc-377055, 1:200), c-fos (Abcam, Cat#ab222699, 1:1000), TH (Merck, Cat#MAB318, 1:200) diluted in hybridization solution were then incubated with the NAc sections at 4°C overnight.

Techniques: Virus, shRNA, Software

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Expression of AKAP150 upregulated in the NAc after naloxone-precipitated morphine withdrawal (A–C) Schematic of the three-compartment apparatus applied for the CPA test. (B and C) Experimental timeline of the morphine withdrawal physical signs test (B) and the morphine withdrawal CPA model (C). (D) Morphine withdrawal physical signs test [jumps (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), paw tremor (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, Mann-Whitney U test, n = 10)] and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly increased after morphine withdrawal in WT mice. (E) Less time was spent in the paired compartment after the morphine withdrawal CPA model was established. CPA aversion score was significantly reduced after morphine withdrawal in WT mice (∗∗∗ P <0.001, t test, n = 10). (F) Mean velocity showed no significant difference before and after morphine withdrawal (Pre-paired, p = 0.6961, t test; Post-paired, p = 0.7676, t test; n = 10). (G–I) AKAP150 mRNA (G) and protein expression (H and I) were upregulated in morphine withdrawal model by PCR, IHC, and WB test (∗∗∗ P <0.001, t test, n = 6). (J). IHC showing c-fos expression was upregulated after morphine withdrawal. Scale bar, 100μm. (K) Representative images of GFP-labeled dendrites and the statistical data for density of dendritic spines, demonstrating the density of dendritic spine was significantly higher after morphine withdrawal (∗∗∗ P <0.001, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and CPA score in WT mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗∗ P <0.01, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, t test, n = 10) were decreased, and morphine withdrawal score (∗∗ P <0.01, Mann-Whitney U test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in WT mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in WT mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in WT mice. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in WT mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Viruses used in the study

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: shRNA

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and aversion score in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased, and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice morphine withdrawal model. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: AKAP150 was highly expressed in D1R-MSNs and D2R-MSNs (A) UMAP plot of 35,878 single cells grouped into 19 major cell types. Each dot represents one single cell, colored according to cell type. (B) Feature plot of the normalized expression of AKAP150 gene for each cell type and the depth of color from gray to blue represents low to high expression. (C) Violin plots showed the normalized expression of AKAP150 gene (rows) in each cell cluster (columns). Cell clusters and the expression level of AKAP150 are indicated at the x and y axis, respectively. (D) Violin plots showed the normalized expression of AKAP150 gene (rows) from one time morphine-treated mice and saline-treated mice (columns) in D1R-MSN cluster (left ), D2R-MSN cluster (middle ) and GABA_other cluster (right ). Treat group and the expression level of AKAP150 are indicated at the x and y axis, respectively. p values are derived from two-sided Wilcoxon test. (E) Level of AKAP150 mRNA relative expression was not significantly changed after one time treatment of morphine compared with saline (N.S. = no significance, p > 0.05, t test, n = 6). (F and G) IHC showed expression of AKAP150 in D1 (F) and D2 (G) receptor positive neuron in the naloxone-precipitated morphine withdrawal mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Expressing, Saline, Derivative Assay

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in WT mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in WT mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗∗ P <0.001, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in WT mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic responses including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). (G) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. (H) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Knockdown, Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Activity of AKAP150 expressed in D1R-MSNs and D2R-MSNs in NAc received mediation from VTA (A and B) AAV1-TH-Cre in VTA promoted the expression of CMV-DIO-shRNA-EGFP in NAc and relieved the morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗ P <0.05, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower (A) as well as the CPA scores (∗∗∗ P <0.001, t test, n = 10) (B). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in the NAc mediated by VTA (∗∗ P <0.01, t test, n = 6). (D and E) Inhibition of AKAP150 in D1R-MSNs receiving projections from VTA relieved the morphine withdrawal aversion (∗∗ P <0.01, t test, n = 10) (E) without relieving morphine withdrawal somatic signs (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10) (D). (F) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D1R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). (G and H) Inhibition of AKAP150 in D2R-MSNs receiving projections from VTA relieved the morphine withdrawal somatic signs [jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, t test, n = 10), loss of body weight (∗∗ P <0.01, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10)] (G) without relieving the morphine withdrawal aversion (N.S. = no significance, p > 0.05, t test, n = 10) (H). (I) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D2R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Activity Assay, Expressing, shRNA, MANN-WHITNEY, Knockdown, Inhibition

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet:

Article Snippet: AKAP150 flox/flox (AKAP150 fl/fl ) mice, which had been used in previous research, , were purchased from the Jackson Laboratory.

Techniques: Virus, shRNA, Software

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Expression of AKAP150 upregulated in the NAc after naloxone-precipitated morphine withdrawal (A–C) Schematic of the three-compartment apparatus applied for the CPA test. (B and C) Experimental timeline of the morphine withdrawal physical signs test (B) and the morphine withdrawal CPA model (C). (D) Morphine withdrawal physical signs test [jumps (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), paw tremor (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, Mann-Whitney U test, n = 10)] and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly increased after morphine withdrawal in WT mice. (E) Less time was spent in the paired compartment after the morphine withdrawal CPA model was established. CPA aversion score was significantly reduced after morphine withdrawal in WT mice (∗∗∗ P <0.001, t test, n = 10). (F) Mean velocity showed no significant difference before and after morphine withdrawal (Pre-paired, p = 0.6961, t test; Post-paired, p = 0.7676, t test; n = 10). (G–I) AKAP150 mRNA (G) and protein expression (H and I) were upregulated in morphine withdrawal model by PCR, IHC, and WB test (∗∗∗ P <0.001, t test, n = 6). (J). IHC showing c-fos expression was upregulated after morphine withdrawal. Scale bar, 100μm. (K) Representative images of GFP-labeled dendrites and the statistical data for density of dendritic spines, demonstrating the density of dendritic spine was significantly higher after morphine withdrawal (∗∗∗ P <0.001, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and CPA score in WT mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗∗ P <0.01, Mann-Whitney U test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗∗ P <0.001, t test, n = 10) were decreased, and morphine withdrawal score (∗∗ P <0.01, Mann-Whitney U test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in WT mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in WT mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in WT mice. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in WT mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Viruses used in the study

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: shRNA

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Knockdown of AKAP150 expression in the NAc relieved morphine withdrawal somatic response and aversion score in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs including jumps (∗∗∗ P <0.001, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗∗ P <0.001, t test, n = 10), wet-dog shake (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), diarrhea (∗∗∗ P <0.001, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased, and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice. (B) Aversion score was improved after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗ P <0.01, t test, n = 10). (C) IHC showed c-fos expression was downregulated after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice morphine withdrawal model. Scale bar, 100μm. (D) Density of dendritic spines was significantly decreased after knockdown of AKAP150 expression in NAc in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 6). Scale bar, 10μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: AKAP150 was highly expressed in D1R-MSNs and D2R-MSNs (A) UMAP plot of 35,878 single cells grouped into 19 major cell types. Each dot represents one single cell, colored according to cell type. (B) Feature plot of the normalized expression of AKAP150 gene for each cell type and the depth of color from gray to blue represents low to high expression. (C) Violin plots showed the normalized expression of AKAP150 gene (rows) in each cell cluster (columns). Cell clusters and the expression level of AKAP150 are indicated at the x and y axis, respectively. (D) Violin plots showed the normalized expression of AKAP150 gene (rows) from one time morphine-treated mice and saline-treated mice (columns) in D1R-MSN cluster (left ), D2R-MSN cluster (middle ) and GABA_other cluster (right ). Treat group and the expression level of AKAP150 are indicated at the x and y axis, respectively. p values are derived from two-sided Wilcoxon test. (E) Level of AKAP150 mRNA relative expression was not significantly changed after one time treatment of morphine compared with saline (N.S. = no significance, p > 0.05, t test, n = 6). (F and G) IHC showed expression of AKAP150 in D1 (F) and D2 (G) receptor positive neuron in the naloxone-precipitated morphine withdrawal mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Expressing, Saline, Derivative Assay

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in WT mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in WT mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗∗ P <0.001, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗∗ P <0.01, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in WT mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in WT mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Evaluation of morphine withdrawal score and aversion score after knockdown of AKAP150 in D1R-MSNs or D2R-MSNs in AKAP150 fl/fl mice (A) Morphine withdrawal somatic signs and morphine withdrawal score showed no significant differences after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (B) Aversion score was improved after knockdown of AKAP150 expression in D1R-MSNs in AKAP150 fl/fl mice (∗∗∗ P <0.001, t test, n = 10). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D1R-MSNs (∗∗ P <0.01, t test, n = 6). (D) Morphine withdrawal somatic responses including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗ P <0.05, t test, n = 10), teeth chattering (∗∗ P <0.01, t test, n = 10), wet-dog shake (∗∗ P <0.01, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10) were decreased and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice. (E) Aversion score showed no significant differences after knockdown of AKAP150 expression in D2R-MSNs in AKAP150 fl/fl mice (N.S. = no significance, p > 0.05, t test, n = 10). (F) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in D2R-MSNs (∗∗ P <0.01, t test, n = 6). (G) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. (H) IHC demonstrated double labeling of AKAP150 and D1-MSNs in the AKAP150 fl/fl mice. Scale bar, 100μm. Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Knockdown, Expressing, MANN-WHITNEY, Labeling

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet: Activity of AKAP150 expressed in D1R-MSNs and D2R-MSNs in NAc received mediation from VTA (A and B) AAV1-TH-Cre in VTA promoted the expression of CMV-DIO-shRNA-EGFP in NAc and relieved the morphine withdrawal somatic signs including jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗ P <0.05, t test, n = 10), diarrhea (∗ P <0.05, Mann-Whitney U test, n = 10), and loss of body weight (∗ P <0.05, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10) was significantly lower (A) as well as the CPA scores (∗∗∗ P <0.001, t test, n = 10) (B). (C) Density of dendritic spines was significantly decreased after the knockdown of AKAP150 in the NAc mediated by VTA (∗∗ P <0.01, t test, n = 6). (D and E) Inhibition of AKAP150 in D1R-MSNs receiving projections from VTA relieved the morphine withdrawal aversion (∗∗ P <0.01, t test, n = 10) (E) without relieving morphine withdrawal somatic signs (N.S. = no significance, p > 0.05, t test except diarrhea using Mann-Whitney U test, n = 10) (D). (F) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D1R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). (G and H) Inhibition of AKAP150 in D2R-MSNs receiving projections from VTA relieved the morphine withdrawal somatic signs [jumps (∗ P <0.05, t test, n = 10), paw tremor (∗∗ P <0.01, t test, n = 10), teeth chattering (∗ P <0.05, t test, n = 10), wet-dog shake (∗∗ P <0.01, Mann-Whitney U test, n = 10), diarrhea (∗ P <0.05, t test, n = 10), loss of body weight (∗∗ P <0.01, t test, n = 10), and morphine withdrawal score (∗∗∗ P <0.001, t test, n = 10)] (G) without relieving the morphine withdrawal aversion (N.S. = no significance, p > 0.05, t test, n = 10) (H). (I) Density of dendritic spines was significantly decreased after inhibition of AKAP150 in D2R-MSNs receiving projections from VTA (∗∗ P <0.01, t test, n = 6). Data are shown as the mean ± SEM.

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Activity Assay, Expressing, shRNA, MANN-WHITNEY, Knockdown, Inhibition

Journal: iScience

Article Title: AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

doi: 10.1016/j.isci.2023.108227

Figure Lengend Snippet:

Article Snippet: Primary antibodies against β-actin (Proteintech, Cat# 66009-1, 1:5000) and AKAP150 (Santa Cruz, Cat# sc-377055, 1:200) were then used to incubate the PVDF membranes at 4°C overnight.

Techniques: Virus, shRNA, Software